SARS corona virus peptides recognized by antibodies in the sera of convalescent cases.
Identifieur interne : 001797 ( Main/Exploration ); précédent : 001796; suivant : 001798SARS corona virus peptides recognized by antibodies in the sera of convalescent cases.
Auteurs : Jian-Ping Guo [Canada] ; Martin Petric ; William Campbell ; Patrick L. McgeerSource :
- Virology [ 0042-6822 ] ; 2004.
Descripteurs français
- KwdFr :
- Adulte d'âge moyen, Anticorps antiviraux (sang), Cellulose, Convalescence, Données de séquences moléculaires, Femelle, Humains, Maladie aigüe, Mâle, Peptides (immunologie), Peptides (sang), Peptides (synthèse chimique), Protéines virales (immunologie), Sujet âgé, Syndrome respiratoire aigu sévère (sang), Séquence d'acides aminés, Tests de neutralisation, Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
- MESH :
- immunologie : Peptides, Protéines virales, Vaccins antiviraux, Virus du SRAS.
- sang : Anticorps antiviraux, Peptides, Syndrome respiratoire aigu sévère.
- synthèse chimique : Peptides.
- Adulte d'âge moyen, Cellulose, Convalescence, Données de séquences moléculaires, Femelle, Humains, Maladie aigüe, Mâle, Sujet âgé, Séquence d'acides aminés, Tests de neutralisation.
English descriptors
- KwdEn :
- Acute Disease, Aged, Amino Acid Sequence, Antibodies, Viral (blood), Cellulose, Convalescence, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Neutralization Tests, Peptides (blood), Peptides (chemical synthesis), Peptides (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (blood), Viral Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Peptides.
- chemical , chemical synthesis : Peptides.
- chemical , immunology : Peptides, Viral Proteins, Viral Vaccines.
- blood : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- Acute Disease, Aged, Amino Acid Sequence, Cellulose, Convalescence, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Neutralization Tests.
Abstract
We synthesized on cellulose membranes 4942 ten-amino-acid peptides which included all of the sequences predicted for the severe acute respiratory syndrome (SARS) corona virus. We probed these membranes with four pairs of acute and convalescent sera from recovered SARS cases. We correlated positively reacting peptides with the in vitro SARS-CoV neutralizing activity of the samples. We found that convalescent sera with high neutralizing activity recognized exclusively only a limited number of peptides on the membranes. This suggests that antibodies against the epitopes represented by these peptides could be responsible for much of the SARS-CoV neutralizing activity. The findings have implications for monitoring humoral responses to SARS-CoV as well as for developing a successful SARS vaccine.
DOI: 10.1016/j.virol.2004.04.017
PubMed: 15207612
Affiliations:
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Le document en format XML
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Mcgeer</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute Disease</term><term>Aged</term><term>Amino Acid Sequence</term><term>Antibodies, Viral (blood)</term><term>Cellulose</term><term>Convalescence</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Peptides (blood)</term><term>Peptides (chemical synthesis)</term><term>Peptides (immunology)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (blood)</term><term>Viral Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte d'âge moyen</term><term>Anticorps antiviraux (sang)</term><term>Cellulose</term><term>Convalescence</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Humains</term><term>Maladie aigüe</term><term>Mâle</term><term>Peptides (immunologie)</term><term>Peptides (sang)</term><term>Peptides (synthèse chimique)</term><term>Protéines virales (immunologie)</term><term>Sujet âgé</term><term>Syndrome respiratoire aigu sévère (sang)</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Peptides</term><term>Viral Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Peptides</term><term>Protéines virales</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Peptides</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acute Disease</term><term>Aged</term><term>Amino Acid Sequence</term><term>Cellulose</term><term>Convalescence</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte d'âge moyen</term><term>Cellulose</term><term>Convalescence</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Humains</term><term>Maladie aigüe</term><term>Mâle</term><term>Sujet âgé</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We synthesized on cellulose membranes 4942 ten-amino-acid peptides which included all of the sequences predicted for the severe acute respiratory syndrome (SARS) corona virus. We probed these membranes with four pairs of acute and convalescent sera from recovered SARS cases. We correlated positively reacting peptides with the in vitro SARS-CoV neutralizing activity of the samples. We found that convalescent sera with high neutralizing activity recognized exclusively only a limited number of peptides on the membranes. This suggests that antibodies against the epitopes represented by these peptides could be responsible for much of the SARS-CoV neutralizing activity. The findings have implications for monitoring humoral responses to SARS-CoV as well as for developing a successful SARS vaccine.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Campbell, William" sort="Campbell, William" uniqKey="Campbell W" first="William" last="Campbell">William Campbell</name><name sortKey="Mcgeer, Patrick L" sort="Mcgeer, Patrick L" uniqKey="Mcgeer P" first="Patrick L" last="Mcgeer">Patrick L. Mcgeer</name><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name></noCountry><country name="Canada"><noRegion><name sortKey="Guo, Jian Ping" sort="Guo, Jian Ping" uniqKey="Guo J" first="Jian-Ping" last="Guo">Jian-Ping Guo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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